Auragen is a Singapore based genomics service provider. Our prestigious customes in Singapore include National Cancer Center, National University of Singapore, Duke NUS, KK Women and Children Hospital, SingHealth, Cancer Science Institute and Insitute of Molecular Cell Biology, and in Malaysia - Institute of Medical Research and University Malaya Medical Center.
Our mission is to enable clinical and academic researchers to advance their research by providing state-of-the-art technologies and reliable genomic services.
Our vision is to be the leading genomic service provider in Southeast Asia.
We share our vision with our partners TheragenETEX and NCCS-IGP to provide researchers with quality data, fast turnaround time and cost-effective price.
DNA / RNA is the foundation to contemporary molecular biology that provides the basis for detailed genomics research. The correct choice of DNA / RNA extraction method for samples prior to assay and analysis have myriad of benefits. We offer high quality DNA and RNA extraction from a variety of sample sources (Blood, Saliva, Cells, Fresh tissue, Frozen tissue and FFPE) using automated platforms (QiAcube and QiAsymphony).
Whole Genome Sequencing (WGS) application enables discovery and identification of singe nucleotide variants (SNV), structural variants (SV), and copy number variants (CNV) in coding and non-coding regions. We offer standard coverage (30X) and deep coverage (60X and 90X) on Illumina HiSeq X Ten sequencer. Appropriate kits for Library preparation, standardized workflow and analytical pipelines allow us to offer reliable and scalable WGS.
Whole Transcriptome Sequencing or RNA Sequencing (RNA Seq) application enables gene expression, differential expression, allele specific mutation detection, discovery of fusion transcripts and splice junctions. We offer sequencing on Illumina HiSeq 2500 sequencer to depths from 50 million reads and above. Strand specific kits for Library preparation, standardized workflow and analytical pipelines allow us to offer reliable RNA Seq.
Whole Exome Sequencing (WES) provides an excellent alternative to Whole Genome Sequencing (WGS). Its application enables variant detection across a variety of genetic diseases and cancer. We offer a choice of Agilent or Illumina or other vendors Exome capture kits for Library preparation, sequencing on Illumina HiSeq 4000 sequencer with various coverages (from 100X and above) and analytical pipelines for reliable WES.
Targeted Bisulphite Sequencing (Human) enables researchers to focus on differentially methylated region (DMR), promoters, CpG islands, shores and shelves, DNase1 hypersensitive sites and RefGenes. Whole Genome Bisulphite Sequencing (WGBS) application enables evaluating methylation state of almost every CpG sites of the genome. We offer Targeted Bisulphite sequencing or WGBS on Illumina HiSeq 4000 or HiSeq X Ten sequencers.
We offer sequencing-only services (Lane Seq or Lane Rental) for constructed libraries - single end or paired end on individual or multiplexed libraries with your choice of read length, flow cell and Illumina HiSeq sequencers. This allows the researchers to flexibly construct their own libraries, use their own indices or barcoded adapters. Lane Seq services includes QC on Bioanalyzer and QPCR quantification. FASTQ data and FASTQC reports are provided.
Microarray solutions enable researchers to understand the complex genetic architecture behind genetic diseases and cancer. Our process includes RNA / DNA sample processing and multiple QC steps that guarantees data quality for analysis. With Illumina microarray Platforms, we offer genotyping, copy number variation (CNV) and methylation array services that enable reproducible, rapid, and accurate microarray analyses.
Nanostring technology enables researchers to validate their NGS or Microarray data. We offer Nanostring nCounter for gene expression analysis of defined gene sets, miRNA Profiling, simultaneous quantification of mRNA and miRNA and Analysis of DNA (CNVs, ChIP, and Gene Fusion). It can multiplex up to 800 hundreds of targets in a single reaction with low amount of input material (100 ng of total RNA or 500 ng of gDNA per sample).
Good DNA / RNA extraction form various sample sources (Blood, Saliva, Cells, Fresh tissue, Frozen tissue and FFPE) can make all the difference when it comes to getting quality genomic data. DNA or RNA extraction steps from sample lysis to elution of highly pure DNA or RNA are seamlessly integrated and automated by QiAcube or QiAsymphony.
NGS technology (Illumina, Pacbio and Life) enables researchers rapidly sequence whole genomes, deeply sequence target regions, discover novel RNA variants and splice sites, quantify mRNAs for gene expression analysis, analyze epigenetic factors, study rare somatic variants in cancer samples and investigate microbial diversity in humans.
DNA Microarray technology (Illumina iScan) enables researchers to explore genetic variants such as single nucleotide polymorphisms (SNPs) and large structural changes, Investigate methylation patterns quantitatively across the genome and epigenetic changes in cancer. Its applications are widely used in gene discovery, disease diagnosis.
The Nanostring (nCounter) system provides a simple workflow optimized for challenging samples such as FFPE. It can multiplex up to 800 hundreds of targets in a single reaction with low amount of input material (100 ng of total RNA or 500 ng of gDNA per sample) enabling researchers to analyze RNA samples directly from lysate or from FFPE samples.